Molecular Cloning and Sequence Analysis of Bubaline Lactoferrin Promoter

Abstract

The proximal promoter for bubaline lactoferrin-encoding gene has been isolated, cloned and sequenced. A 468 b fragment of the 5′ flanking region of the Pactoferrin gene was PCR amplified and cloned into pUC18 vector. Sequence analysis of the amplified fragment revealed the presence of one TATA box, one TATA like element, two GC boxes and one motif resembling CAMP response element (CRE) in this reion. Bubaline lactoferrin romoter shares 93%, 53%, 52% and 48% homoPogy with cattle, pig, mouse and human lactoferrin 5′ flanking region, respectively.

Key Words:

• Lactoferrin
• PCR
• promoter