Cloning and Characterization of a cDNA Encoding Catalase in Wheat

Abstract

We determined the nucleotide sequence of a cDNA encoding the catalase (CAT) isolated from wheat (Triticum aestivum L.). The deduced amino acid sequence suggests that this wheat catalase isozyme shared higher amino acid homology with group I CATs of barley CAT-1, rice CAT B and maize CAT-1 and CAT-2 but lower homology with group II CATs of barley CAT-2, rice CAT A and maize CAT-3. Both group I and II specific sequences of Ser-Arg-Leu and Ser-Ser-Ser, respectively considered as peroxisomal targeting signals were found first in monocot plant of wheat. Functionally important amino acids at active center and heme-binding sites detected in all other plant catalase were conserved.

Key Words:

• activated oxygen
• catalase
• low temperature
• rice (Oryza sativa L.)
• wheat (Triticum aestivum L).