21
Views
2
CrossRef citations to date
0
Altmetric
Original Article

cDNA Cloning and Chromosomal Mapping of Mouse BH-Protocadherin

, , , , &
Pages 43-47 | Received 29 Jun 1998, Published online: 11 Jul 2009
 

Abstract

We isolated and determined the sequence of cDNA encoding mouse BH-protocadherin (BH-Pcdh) from heart. It encodes a 1069 amino acids (aa) polypeptide exhibiting an overall 97% identity with human BH-Pcdh-a and 83% identity with Xenopus NF-protocadherin. We also determined the alternatively spliced cytoplasmic tail sequence. The cytoplasmic tail of mouse BH-Pcdh-b is short (2 aa) compared with that of human BH-Pcdh-b (14 aa). The cytoplasmic tail of mouse BH-Pcdh-c showed 100% aa identity with that of human BH-Pcdh-c except for a 8-amino-acid insertion. Northern blot analysis revealed two major transcripts were expressed in brain and heart. Mouse BH-Pcdh-a mRNA was detected as a single band of approximately 5.5-kb. The mRNA level of mouse BH-Pcdh-a and -c were persistently detected by RT-PCR in developmental process of brain and heart, but that of mouse BH-Pcdh-b was elevated only in fetus and neonatal stage of brain. The chromosomal location of the mouse BH-Pcdh gene was determined as 5C3-D using fluorescence in situ hybridization.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.