Impaired Transendothelial Migration of B-CLL Lymphocytes: a Defect Linked to Low L-Selectin Expression

Abstract

The emigration of lymphocytes from blood into lymph nodes is regulated by the expression of the adhesion molecule, L-selectin on the lymphocyte surface which arrests the rolling of the cell on the vessel wall and allows firmer adhesive interactions to develop. The expression of L-selectin on B-CLL lymphocytes is less than half that on normal lymphocytes and this difference correlates with an impaired capacity of B-CLL lymphocytes to migrate beneath a monolayer of human umbilical vein endothelial cells. Both the B-cell and T-cell lymphocytes from normal subjects and B-CLL patients show down-regulation of L-selectin and CD23 after transendothelial migration. The reduced expression of L-selectin on B-CLL lymphocytes leads to a relative “trapping” of these cells in the vascular space and is one factor contributing to the elevation of peripheral lymphocyte count.

Key Words:

• impaired transendothelial migration
• B-CLL
• L-Selectin expression
• chronic lymphocytic leukemia