Differential protein analysis of lymphocytes between children with acute lymphoblastic leukemia and healthy children

Abstract

We identified differential proteins in lymphocytes between patients with childhood acute lymphoblastic leukemia (c-ALL) and healthy children. Samples of bone marrow lymphocytes from children with c-ALL and peripheral blood lymphocytes from healthy children were collected, and total proteins were extracted and separated from these samples followed by two-dimensional gel electrophoresis for comparative analysis. The differential protein spots in c-ALL cells were digested in situ, and then analyzed with matrix-assisted laser desorption ionization/time of flight mass spectrometry (MALDI-TOF-MS) followed by identification using the relevant database. Fifteen differential expression proteins were obtained by comparative proteomics analysis. Of the 15 differential proteins, eight were identified. Of the eight proteins, two had high expression and six low expression in c-ALL cells. The eight differential proteins are expected to become new diagnostic markers and drug targets for c-ALL.

Keywords::

• Acute lymphoblastic leukemia
• child
• proteomics
• two-dimensional gel electrophoresis
• mass spectrum

Acknowledgements

We thank Professor Yu-feng Liu of the Pediatric Department of the First Affiliated Hospital of Zhengzhou University. The work of the authors was supported by grants from the Pediatric Hematology and Oncology Research Institute and the research council of Zhengzhou University. This work was supported by the Nature Science Foundation of the education department of He Nan province in China (No. 2010A320028).

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