Expression of Deoxycytidine Kinase (dCK) Gene in Leukemic Cells in Childhood: Decreased Expression of dCK Gene in Relapsed Leukemia

Abstract

Competitive RT-PCR was used to determine the quantitative variation in the expression of deoxycytidine kinase (dCK) gene in childhood leukemic cells. The degree of dCK gene expression varied over a 50-fold range. In two cases in which both primary and relapsed leukemic cells were analysed, decreased expression of dCK gene was found in relapsed leukemic cells. The sequence variation analysis using bisbenzimide/polyethylene glycol electrophoresis demonstrated no sequence alteration of dCK cDNA in all cases. These results indicate that the expression of dCK gene varies in patients and suggests decreased expression of the dCK gene as one of the mechanisms responsible for clinical resistance to ara-C.

Key Words:

• 1-β-D-arabinofuranosylcytosine
• deoxycytidine kinase gene
• RT-PCR
• leukemia