Expression of CD44 Isoforms on Isolated Bone Marrow Plasma Cells and Peripheral CD19⁺ B Cells of Patients with Multiple Myeloma and Healthy Individuals

Abstract

The expression of certain isoforms of CD44 was shown to correlate with aggressiveness and metastatic potential of various tumour types. We analysed the expression of the adhesion molecule CD44 and its variant domains (v6, v7, v7/8, v10) on isolated bone marrow (BM) plasma cells and peripheral blood (PBL) CD19⁺ B cells of 21 patients with MM and 15 healthy donors. B cells and plasma cells were isolated by immunomagnetic sorting and analysed by two-colour flow cytometry. The expression of CD44 isoforms was significantly higher on PBL B cells of patients with MM than in healthy controls. The elevated expression of CD44 isoforms (v6, v7/8, v10) on PBL B cells correlated with reduced overall survival in MM. CD44 isoforms were more strongly expressed on “larger”, activated B cells. Furthermore, CD44 isoforms were found to be simultaneously expressed with CD38^hi and CD56 on both, B lymphocytes and plasma cells of patients with MM. The determination of CD44 isoforms on circulating B cells may be helpful in defining prognostically unfavourable subgroups in MM.

Key Words:

• multiple myeloma
• CD44 isoforms
• CD19⁺ B cells
• plasma cells
• survival