K Thompson, P Dockery, RW Horobin. Predicting and avoiding subcellular compartmentalization artifacts arising from acetoxymethyl ester calcium imaging probes. The case of fluo-3 AM and a general account of the phenomenon including a problem avoidance chart. Biotechnic & Histochemistry 2012;87(7):468–483.
Spacing errors in the tables may interfere with their interpretation. The tables have been re-formatted and reproduced below. We regret any confusion that may have been caused by these errors.
Table 1. Key physicochemical properties and predicted intracellular localizations of fully, partially and totally de-esterified species of various widely used calcium probes
Table 2. Decision logic table based on structured parameters and published QSAR models for predicting membrane permeability and binding to plasma membrane of calcium probes
Table 3. Decision logic table based on numerical structure parameters and published QSAR models for predicting compartmentalization of calcium probes in cell organelles. For models and parameters, see Horobin (2010)
Table 4. Examples of compartmentalization following application of the AM ester forms of some commonly used calcium probes