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Abstract
Wertheim's hydrosulfite-Schiff reagent (Hasten and Burton, Stain Techn., 34, 289. 1959) gave excellent results for the nucleal reaction. Its use for the periodic acid-Schiff reaction in Paramecium, Entamoeba, Acanthamoeba, Eimeria and Myxosporidia was of no significance, since PAS positive material (as shown by the usual sulfite-Schiff reagent) remained unstained.