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Original Article

Acceleration of the Movat Pentachrome One Stain by Heat

Pages 257-259 | Published online: 12 Jul 2009
 

Abstract

The conventional staining time for Movat's pentachrome I stain (Arch. Path., 60: 289–295, 1955) was shortened from about 18–19 hr to about 2.5 hr. The ammoniated alcohol and the resorcin-fuchsin staining baths were heated to 56 C. All other steps in the technic were performed at 25–27 C. Staining properties of paraffin sections of many types of tissue fixed in formalin, formol-sublimate-acetic, or in Bouin's fluid, showed that staining with resorcin-fuchsin at the elevated temperature gave the same results as staining at room temperature.

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