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Stain Technology Volume 46, 1971 - Issue 2
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Original Article

Saponification of Methylated Mucosubstances at Low Temperatures

Tapani E. Sorvari Department of Pathological Anatomy, University of Turku, Finland
&
Peter J. Stoward Department of Anatomy, University of Dundee, Dundee, Scotland
Pages 49-52 | Published online: 12 Jul 2009
 
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Abstract

A fresh 1% solution of KOH in 70% ethanol in 2 hr at 2-4 C restores basophilia to methylated acid mucosubstances satisfactorily without detaching or damaging tissue sections. A 0.5% solution of Ba(OH)2 under the same conditions gives results nearly as good, but NaOH and KMnO4 are unsatisfactory.

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