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Stain Technology Volume 50, 1975 - Issue 5
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Original Article

A Decalcification Method for Ultrastructure of Echinoderm Tissues

H. F. Dietrich Department of Biology, University of Victoria, Victoria, BC, Canada
&
A. R. Fontaine Department of Biology, University of Victoria, Victoria, BC, Canada
Pages 351-354 | Published online: 12 Jul 2009
 
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Abstract

Decalcification of echinodcrm tissues for electron microscopy can be achieved after glutaraldehyde-osmium firation by treatment with a 1:1 mixture of 2% ascorbic acid and 0.3 M NaCI for 12-24 hours. Electron photomicrographs of material decalcified by this procedure are superior to those from EDTA-treated tiasue and show few of the deleteriour effects produced by EDTA.

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