Improved Diethylene Glycol Distearate Embedding Wax

Abstract

Diethylene glycol distearate wax and cellulose caprate resin, 4:1 respectively by weight, were melted together at 75 C for five hours with occasional stirring. The resin tempered the extreme brittleness of the wax without softening it, and raised the melting point only one degree to 50 C. Fixed plant tissues were dehydrated in ethanol, cleared in xylene, and infiltrated with wax. Modified diethylene glycol distearate was easier to trim and shape, and formed flat sections more consistently than the pure wax. Sections were cut singly on Ralph knives with attached water pools on an ultramicrotome. Sectionability was excellent at 2-3 jam, variable at 1.0 μm, but impossible at 0.5 μm. Sections were transferred onto water drops on slides, dried, dewaxed, stained, and coverglasses applied as in the paraffin method. Histological features of plant tissues were much sharper in modified diethylene glycol distearate sections than in paraffin sections, and were similar to plastic sections.