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Abstract
The analysis of sister chromatid exchanges using the 5-bromodeoxyuridine probe method has become popular in cytological studies today. Many techhiques have been developed to stain the sister chromatids differentially. From our experience, we feel that the method of fluorescence plus Giemsa (FPG), originally developed by Perry and Wolff (1974) and later improved by Goto et al. (1978), gives the most satisfactory results. However, the staining protocols drafted by these authors are not as simple as the one currently practiced in our laboratory. Using metaphase chromosomes of Chinese hamster ovary cells which have replicated two cycles in culture medium containing 10 μM of 5-bromodeoxyuridine as an example, we carry out the simplified FPG method by the following steps.