Permanent Preservation of Whole Alizarin Red S Skeletons by Clearing and Embedding in Polyester Resins

Daniel Chappard L.B.T.O. Laboratory of Bone Biology, GIP “Exercise” North Hospital, University of Saint Etienne, 42277, Saint-Priest-en-Jarez, France

Christian Alexandre L.B.T.O. Laboratory of Bone Biology, GIP “Exercise” North Hospital, University of Saint Etienne, 42277, Saint-Priest-en-Jarez, France

Sabine Palle L.B.T.O. Laboratory of Bone Biology, GIP “Exercise” North Hospital, University of Saint Etienne, 42277, Saint-Priest-en-Jarez, France

Georges Riffat L.B.T.O. Laboratory of Bone Biology, GIP “Exercise” North Hospital, University of Saint Etienne, 42277, Saint-Priest-en-Jarez, France

Abstract

A one-step clearing and embedding procedure for alizarin red S stained skeletons is described. Embryos are fixed in formalin, skinned and eviscerated. After staining in a 10 mg/liter solution of alizarin red S in 5% aqueous KOH, specimens are dehydrated in a graded series of acetone-polyester monomer solutions. Finally, the specimens are embedded at room temperature in the polyester resin. A special reusable metallic mold is described for embedment of large fetuses. Specimens previously cleared in glycerol can be processed with this method.

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Permanent Preservation of Whole Alizarin Red S Skeletons by Clearing and Embedding in Polyester Resins

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Permanent Preservation of Whole Alizarin Red S Skeletons by Clearing and Embedding in Polyester Resins

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