Cerium-Based Demonstration of Phosphatas Activity in Plastic-Embedded Sections: A Comparison with Conventional Methods

Abstract

Cerium-based methods have been used for the demonstration of seve phatases at alkaline, acid and neutral pH in low temperature acetone-fixed, plastic-e sections. At alkaline pH calcium is used as capturing agent and the precipitated phosphate converted to cerium phosphate. At neutral and acid pH cerium is used d capturing agent. Cerium phosphate is subsequently visualized using the H₂C₂DAB n: comparison has been made with conventional calcium-cobalt and lead methods. It; that calcium-cobalt methods are more susceptible to improvement than lead methods