Differential Silver Enhanced Double Labeling in Immunoelectron Microscopy

Abstract

A two-sided double labeling method using protein A gold was used to demonstrate the presence of two hormones within the same anterior pituitary cell granule. A single probe size was used for both section faces but one side of the grid was silver enhanced. The use of a single probe size reduced the cost of the study and eliminated the variations in labeling efficiency that result from the use of different probe sizes.

Key Words:

• immunoelectron microscopy
• silver enhancement
• pituitary