Activation of Mouse Oocytes, Fertilization and Development of Mouse Embryos in Vitro after Staining with Trypan Blue or Fluorescein Diacetate

Abstract

The influence of vital staining with trypan blue or fluorescein diacetate on the fertilization of mouse oocytes and the developmental potential of mouse embryos was assessed. Neither stain induced spontaneous activation in mouse oocytes, nor did they impair the in vitro development and implantation of mouse zygotes, two-cell embryos, stressed morulae or blastocysts. However, fertilization and subsequent development of mouse oocytes have been shown to be reduced by vital staining.

Key Words:

• fluorescein diacetate
• trypan blue
• in vitro fertilization
• in vitro development
• mouse oocyte
• mouse embryo