A Rapid Method for Microbial Sample Preparation for the Scanning Electron Microscope

Abstract

A synthetic aromatic polymer has been used for preparing replicas of different microorganisms. This method of preparing highly concentrated (9.6 k) microbiological samples for scanning electron microscopy was compared with a standard method. The micrographs of the replicated samples are satisfactory. This method is rapid, cost effective and produces good results, especially in the case of spore-forming mycelial microorganisms.

Key Words:

• SEM
• microorganism
• spores