RT-PCR Analysis of MMP-9 Expression in Human Articular Cartilage Chondrocytes and Synovial Fluid Cells

K. Tsuchiya Orthopaedic Research Laboratory, R144, Rte 3, Stanford University School of Medicine, 300 Pasteur Dr., Stanford, California, 94305-5341

W. J. Matoney Orthopaedic Research Laboratory, R144, Rte 3, Stanford University School of Medicine, 300 Pasteur Dr., Stanford, California, 94305-5341

T. Vu Veterans Affairs Medical Center, 4005 Miranda Avenue, #154-E, Palo Alto, California, 94304

A. R. Hoffman Veterans Affairs Medical Center, 4005 Miranda Avenue, #154-E, Palo Alto, California, 94304

D. J. Schurman Orthopaedic Research Laboratory, R144, Rte 3, Stanford University School of Medicine, 300 Pasteur Dr., Stanford, California, 94305-5341

R. L. Smith Orthopaedic Research Laboratory, R144, Rte 3, Stanford University School of Medicine, 300 Pasteur Dr., Stanford, California, 94305-5341; Veterans Affairs Medical Center, 4005 Miranda Avenue, #154-E, Palo Alto, California, 94304

Abstract

This report presents a method for analyzing matrix metailoproteinase-9 (92 kD gelatinase) MMP-9 mRNA expression using reverse transcription polymerase chain reaction (RT-PCR). With this method, chondrocytes isolated from small samples of osteoarthritic cartilage showed significantly elevated signal for MMP-9 mRNA compared to normal cartilage. In addition, cells of synovial fluid samples aspirated from osteoarthritic joints also exhibited MMP-9 expression using this technique. RT-PCR proved to be a sensitive method for assessing MMP-9 regulation in osteoarthritic and normal cartilage, and may provide a useful index of arthritic cartilage and synovial fluid cell metabolism when limited tissue is available.

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RT-PCR Analysis of MMP-9 Expression in Human Articular Cartilage Chondrocytes and Synovial Fluid Cells

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RT-PCR Analysis of MMP-9 Expression in Human Articular Cartilage Chondrocytes and Synovial Fluid Cells

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