Selective, sensitive, and rapid liquid chromatography-tandem mass spectrometry method for determination of Guaifenesin in human plasma

Abstract

A simple, sensitive, selective, and rapid liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed and validated for quantitation of guaifenesin in human plasma using guaifenesin-d3 as internal standard (IS). Following solid phase extraction, the analyte was chromatographed using an isocratic mobile phase on a reversed-phase C₁₈ column and analyzed by MS in multiple reaction-monitoring (MRM) mode (positive ion mode). The limit of quantitation for this method was 8 ng/mL and the linear dynamic range was 8–2200 ng/mL.

Keywords::

• Guaifenesin
• LC-MS-MS
• quantitation

Acknowledgements

The authors wish to acknowledge the support and facilities received from Ranbaxy Research Laboratories, Gurgaon, India, for carrying out this work.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.