Renin Gene Expression in Rat Tissues: A New Quantitative Assay Method for Rat Renin mRNA Using Synthetic cRNA

F. Suzuki German Institute for High Blood Pressure Research and Department of Pharmacology, University of Heidelberg, Im Neuenheimer Feld 366, D-6900, Heidelberg, FRG

G. Ludwig German Institute for High Blood Pressure Research and Department of Pharmacology, University of Heidelberg, Im Neuenheimer Feld 366, D-6900, Heidelberg, FRG

W. Hellmann German Institute for High Blood Pressure Research and Department of Pharmacology, University of Heidelberg, Im Neuenheimer Feld 366, D-6900, Heidelberg, FRG

M. Paul German Institute for High Blood Pressure Research and Department of Pharmacology, University of Heidelberg, Im Neuenheimer Feld 366, D-6900, Heidelberg, FRG

K. Lindpaintner German Institute for High Blood Pressure Research and Department of Pharmacology, University of Heidelberg, Im Neuenheimer Feld 366, D-6900, Heidelberg, FRG

K. Murakami Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, 305, Japan

D. Ganten German Institute for High Blood Pressure Research and Department of Pharmacology, University of Heidelberg, Im Neuenheimer Feld 366, D-6900, Heidelberg, FRG

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Abstract

A genomic renin exon 9 fragment was subcloned into vector pSPT18 and used for in vitro transcription to obtain ³²p-labeled rat renin cRNA. Using this cRNA, we established quantitative solution hybridization and specific Northern blotting assays for renin mRNA. We were able to detect renin mRNA in the kidney, heart ventricle and atrium, brain, testis, and adrenal gland of male rats in the concentrations of 430±8.1, 110±1.9, 43±0.9, 64±1.1, 47±0.9 and 11±0.2 pg mRNA/mg of total RNA, respectively.

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Renin Gene Expression in Rat Tissues: A New Quantitative Assay Method for Rat Renin mRNA Using Synthetic cRNA

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Renin Gene Expression in Rat Tissues: A New Quantitative Assay Method for Rat Renin mRNA Using Synthetic cRNA

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