Abstract
The protecting ability of the Piper betle leaves-derived phenol, allylpyrocatechol (APC) against AAPH-induced membrane damage of human red blood cells (RBCs) was investigated. Compared to control, AAPH (50 mM) treatment resulted in significant hemolysis (55%, p < 0.01), associated with increased malondialdehyde (MDA) (2.9-fold, p < 0.001) and methemoglobin (6.1-fold, p < 0.001) levels. The structural deformation due to membrane damage was confirmed from scanning electron microscopy (SEM) images and Heinz bodies formation, while the cell permeability was evident from the K+ efflux (28.7%, p < 0.05) and increased intracellular Na+ concentration (8%, p < 0.05). The membrane damage, due to the reduction of the cholesterol/phospholipids ratio and depletion (p < 0.001) of ATP, 2,3-DPG by ˜44–54% and Na+–K+ ATPase activity (43.7%), indicated loss of RBC functionality. The adverse effects of AAPH on all these biochemical parameters and the resultant oxidative hemolysis of RBCs were significantly reduced by pretreating the cells with APC (7 μM) or α-tocopherol (50 μM) for 1 h, prior to incubation with AAPH.
Acknowledgment
One of the authors (M. K.) is grateful to the University Grants Commission (UGC), Pune for the award of Teacher Fellowship, the principal and N.E.S. Ratnam college of Arts, Science and commerce for the encouragement for research work, and Mr. Prashant Warang, National Institute of Immunohematology (ICMR), K.E.M. Hospital campus, Parel, Mumbai, for technical assistance.
Declaration of interest
The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.