Abortion-prone mating influences placental antioxidant status and adversely affects placental and foetal development

Abstract

Oxidative stress is associated with decreased female fertility and adversely affects prenatal development. Mammalian cells have developed a network of enzymatic and non-enzymatic antioxidant defence systems to prevent oxidative stress. Little attention has been paid to the antioxidative pathways in placentas of normal and disturbed pregnancies, leaving a gap in our knowledge about the role of antioxidants in the control of foeto-placental development. The challenges in studying early human pregnancy can partly be overcome by designing animal models of abnormal pregnancy. We aimed to determine whether the antioxidant status of placentas from the CBA/J × DBA/2 abortion-prone pregnant mice differed from that of normal pregnant mice. The foetal/placental weight ratio was lower in abortion-prone matings compared with that in non-abortion-prone matings. The increased placental malondialdehyde (MDA) content, the end products of lipid peroxidation, with concomitants alterations in placental antioxidants, namely copper-zinc containing superoxide dismutase (SOD1), manganese containing (SOD2), glutathione peroxidases (GPX), glutathione reductase (GR) and catalase (CAT) activities may be involved in placental and foetal growth restriction. We show that placental oxidative stress is linked with poor prenatal development and pregnancy losses in CBA/J × DBA/2 mice matings. This animal model may be useful in the evaluation of nutritional antioxidant therapies for oxidative stress and associated prenatal developmental disorders.

Keywords::

• abortion-prone mating
• oxidative stress
• antioxidants
• placenta
• foeto-placental development

Acknowledgements

We would like to thank Laurence Puillet-Anselme (CHU Grenoble) and Philippe Bolifraud (INRA, Jouy-en-Josas) for their technical assistance. The authors would like to thank the staff of the rodent experimental unit (INRA, Jouy-en-Josas) for outstanding technical help and animal management. We are grateful to Mrs Clare Gaffney for critical reading of the article. The author would like to thank the two anonymous reviewers for their close examination of this article and their useful comments.

Declaration of interest

The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.