EPR and electrochemical quantification of oxygen using newly synthesized para-silylated triarylmethyl radicals

Abstract

Novel silylated triarylmethyl (TAM) radicals based on TAM core CT-03 and their electron paramagnetic resonance (EPR) spectra are evaluated as a function of oxygen concentration. Combination of peak-to-peak linewidth of the EPR signal and electrochemical determination allows designing a method for oxygen quantification in phosphate buffer, dimethylsulfoxide, and dichloromethane, which can be extended to other solvents.

Keywords::

• oximetry
• silylated trityls
• electron paramagnetic resonance
• electrochemistry
• ultramicroelectrode

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

This work was supported by Sanofi in the framework of AVIESAN, the French National Alliance for Life Sciences and Health. The EPR data in this manuscript were obtained using equipment supported jointly by French National Ministry of Research (PPF IRPE), French National Research Agency (ANR IRPE BLAN-06-0351), “Fondation pour la Recherche Médicale” (FRM DGE20061007745), and CNRS (Department of Chemistry and Life Sciences). The authors also thank the analytical sciences department of Sanofi R&D for providing analytical data related to the synthesized compounds.