Advanced search
Publication Cover
Free Radical Research Communications Volume 6, 1989 - Issue 1
Journal homepage
286
Views
1,423
CrossRef citations to date
0
Altmetric
Original Article

Continuous Monitoring of in Vztro Oxidation of Human Low Density Lipoprotein

H. Esterbauer Institute of Biochemistry, University of Graz, Schubertstrasse I, A-8010, Graz, Austria
,
G. Striegl Institute of Biochemistry, University of Graz, Schubertstrasse I, A-8010, Graz, Austria
,
H. Puhl Institute of Biochemistry, University of Graz, Schubertstrasse I, A-8010, Graz, Austria
&
M. Rotheneder Institute of Biochemistry, University of Graz, Schubertstrasse I, A-8010, Graz, Austria
Pages 67-75 | Received 09 Nov 1988, Accepted 15 Nov 1988, Published online: 07 Jul 2009
 
Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days

Abstract

The kinetics of the oxidation of human low densit) lipoprotein (LDL) can be measured continuously by monitoring the change of the 234 nm diene absorption. The time-course shows three consecutive phases, a lag-phase during which the diene absorption increases only weakly. a propagation phase with a rapid increase of the diene absorption and finally a decomposition phase. The increase of the dienes is highly correlated with the increase of MDA or lipid hydroperoxides. The duration of the lag-phase is determined by the endogenous antioxidants contained in LDL (vitamin E. carotenoids. retinylstearate). Water-soluble antioxidants (ascorbic acid. urate) added in micromolar concentrations prolong the lag-phase in a concentration-dependent manner. The determination of the lag-phase is a convenient and objective procedure for determining the susceptibility of LDL from different donors towards oxidation as well as effects of pro-and antioxidants.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related Research Data

Liquid Chromatography
Source: Wiley
Bioavailability and antioxidant capacity of plant extracts rich in polyphenols, given as a single acute dose, in sheep made highly susceptible to lipoperoxidation.
Source: Cambridge University Press (CUP)
Comparison of the effects of fish oil and olive oil on blood lipids and aortic atherosclerosis in Watanabe heritable hyperlipidaemic rabbits.
Source: Cambridge University Press (CUP)
Adiponectin attenuates endothelial dysfunction induced by oxidised low-density lipoproteins.
Source: SAGE Publications
Biomarkers of Oxidative Stress in Plasma and Urine
Source: John Wiley & Sons, Inc.
Antioxidants in relation to lipid peroxidation
Source: Elsevier BV
Application of gas chromatography-mass spectrometry for analysis of isoprostanes: their role in cardiovascular disease.
Source: Walter de Gruyter GmbH
Protective effects of high-density lipoprotein against oxidative stress are impaired in haemodialysis patients
Source: Oxford University Press (OUP)
Ezetimibe's effect on platelet aggregation and LDL tendency to peroxidation in hypercholesterolaemia as monotherapy or in addition to simvastatin.
Source: Wiley
TagSNP analyses of the PON gene cluster: effects on PON1 activity, LDL oxidative susceptibility, and vascular disease
Source: American Society for Biochemistry & Molecular Biology (ASBMB)
Oxidized low-density lipoprotein.
Source: Humana Press
A Caspase-3–Independent Pathway
Source: Ovid Technologies (Wolters Kluwer Health)
Modification of Human Low Density Lipoprotein by Lipid Peroxidation
Source: Springer US
Low Fat and High Monounsaturated Fat Diets Decrease Human Low Density Lipoprotein Oxidative Susceptibility In Vitro
Source: Oxford University Press (OUP)
Oxidized Lipids in the Diet Accelerate the Development of Fatty Streaks in Cholesterol-Fed Rabbits
Source: Ovid Technologies (Wolters Kluwer Health)
Influence of three rapeseed oil-rich diets, fortified with alpha-linolenic acid, eicosapentaenoic acid or docosahexaenoic acid on the composition and oxidizability of low-density lipoproteins: results of a controlled study in healthy volunteers.
Source: Springer Science and Business Media LLC
Mathematical modelling of atheroma plaque formation and development in coronary arteries
Source: The Royal Society
Thermally Oxidized Dietary Fats Increase the Susceptibility of Rat LDL to Lipid Peroxidation but Not Their Uptake by Macrophages
Source: Oxford University Press (OUP)
Antioxidants in tea
Source: Informa UK Limited
Effects of dairy products naturally enriched with cis-9,trans-11 conjugated linoleic acid on the blood lipid profile in healthy middle-aged men
Source: Oxford University Press (OUP)
Moderate Intervention with Carotenoid-Rich Vegetable Products Reduces Lipid Peroxidation in Men
Source: Oxford University Press (OUP)
The effects of cigarette smoking and smoking cessation on high-density lipoprotein functions: implications for coronary artery disease.
Source: SAGE Publications
High-dose consumption of NaCl resulted in severe degradation of lipoproteins associated with hyperlipidemia, hyperglycemia, and infertility via impairment of testicular spermatogenesis
Source: Oxford University Press (OUP)
Vitamin C: antioxidant or pro-oxidant in vivo?
Source: Informa UK Limited
Changes in LDL fatty acid composition as a response to olive oil treatment are inversely related to lipid oxidative damage: The EUROLIVE study.
Source: Informa UK Limited
Original articles
Source: Wiley
Protective effects of 2,3-diaryl-substituted indole-based cyclooxygenase-2 inhibitors on oxidative modification of human low density lipoproteins in vitro
Source: IOS Press
Melatonin and diet-induced obesity
Source: Wiley
Oxidative stress, nutrition and health. Experimental strategies for optimization of nutritional antioxidant intake in humans.
Source: Informa UK Limited
No Antioxidant Effect of Combined HRT on LDL Oxidizability and Oxidative Stress Biomarkers in Treated Post-Menopausal Women
Source: Informa UK Limited
Malondialdehyde-altered protein occurs in atheroma of Watanabe heritable hyperlipidemic rabbits
Source: American Association for the Advancement of Science (AAAS)
Hydroxyl-radical-induced iron-catalysed degradation of 2-deoxyribose. Quantitative determination of malondialdehyde.
Source: Portland Press Ltd.
Almonds and Almond Oil Have Similar Effects on Plasma Lipids and LDL Oxidation in Healthy Men and Women
Source: Oxford University Press (OUP)
Bioavailability and nutritional effects of carotenoids from organically and conventionally produced carrots in healthy men
Source: CABI Publishing
Patients with multiple sclerosis show increased oxidative stress markers and somatic telomere length shortening
Source: Springer Science and Business Media LLC
Oxidative Stress and Protein Oxidation
Source: John Wiley & Sons, Inc.
Lipid oxidation inactivates the anticoagulant function of protein Z-dependent protease inhibitor (ZPI)
Source: American Society for Biochemistry & Molecular Biology (ASBMB)
Effects of Smoking on Serum Levels of Lipid Peroxides and Essential Fat-Soluble Antioxidants:
Source: SAGE Publications
Enhanced Delivery of Rapamycin by V156K-apoA-I High-Density Lipoprotein Inhibits Cellular Proatherogenic Effects and Senescence and Promotes Tissue Regeneration
Source: Oxford University Press (OUP)
The role of altered lipoproteins in the pathogenesis of atherosclerosis
Source: Elsevier BV
Medicinal plants of Cree communities (Québec, Canada): antioxidant activity of plants used to treat type 2 diabetes symptomsThis article is one of a selection of papers published in this special issue (part 2 of 2) on the Safety and Efficacy of Natural Health Products.
Source: Canadian Science Publishing
Investigation of lipid peroxidation in human low density lipoprotein.
Source: Informa UK Limited
In vitro incubation of low-density lipoproteins with inflammatory cells causes enhanced degradation by macrophages in culture.
Source: Springer Science and Business Media LLC
Superoxide-mediated modification of low density lipoprotein by arterial smooth muscle cells
Source: American Society for Clinical Investigation
Treatment with a fixed dose combination antiretroviral therapy drug containing tenofovir, emtricitabine and efavirenz is associated with cardioprotection in high calorie diet-induced obese rats
Source: Public Library of Science (PLoS)
Oxidation of Human Low Density Lipoprotein Initiated by Copper(II)Chloride
Source: Springer US
Oxidation of Low Density Lipoprotein Particles Decreases Their Ability to Bind to Human Aortic Proteoglycans: DEPENDENCE ON OXIDATIVE MODIFICATION OF THE LYSINE RESIDUES
Source: American Society for Biochemistry & Molecular Biology (ASBMB)
Pomegranate juice consumption reduces oxidative stress, atherogenic modifications to LDL, and platelet aggregation: studies in humans and in atherosclerotic apolipoprotein E–deficient mice
Source: Oxford University Press (OUP)
Mulberry (Morus alba L.) Leaves and Their Major Flavonol Quercetin 3-(6-Malonylglucoside) Attenuate Atherosclerotic Lesion Development in LDL Receptor-Deficient Mice
Source: Oxford University Press (OUP)
Proceedings of the British Pharmacological Society. Clinical Pharmacology Section. 14‐16 April 1993. Abstracts.
Source: Wiley
Aminoguanidine Has Both Pro-oxidant and Antioxidant Activity Toward LDL
Source: eScholarship, University of California
In Vivo Formation of 8-Epi-Prostaglandin F2α Is Increased in Hypercholesterolemia
Source: Ovid Technologies (Wolters Kluwer Health)
2,3-Diaryl-substituted indole based COX-2 inhibitors as leads for imaging tracer development
Source: Royal Society of Chemistry (RSC)
Identification of multiple dense LDL subfractions with enhanced susceptibility to in vitro oxidation among hypertriglyceridemic subjects. Normalization after clofibrate treatment.
Source: Ovid Technologies (Wolters Kluwer Health)
Free Radicals and Hearing: Cause, Consequence, and Criteria
Source: Wiley
Pediatric Interventions Using Noninvasive Vascular Health Indices
Source: Ovid Technologies (Wolters Kluwer Health)
Requirement for Estradiol Modification
Source: Ovid Technologies (Wolters Kluwer Health)
Protective effects of magnolol against oxidized LDL-induced apoptosis in endothelial cells
Source: Springer Science and Business Media LLC
QUANTIFICATION AND SIGNIFICANCE OF PROTEIN OXIDATION IN BIOLOGICAL SAMPLES
Source: Informa UK Limited
Decrease in reactive amino groups during oxidation or endothelial cell modification of LDL. Correlation with changes in receptor-mediated catabolism.
Source: Ovid Technologies (Wolters Kluwer Health)
Enhanced susceptibility to in vitro oxidation of the dense low density lipoprotein subfraction in healthy subjects.
Source: Ovid Technologies (Wolters Kluwer Health)
Antioxidant activity of seaweeds
Source: Wiley
Patients with Rheumatoid Arthritis Show Altered Lipoprotein Profiles with Dysfunctional High-Density Lipoproteins that Can Exacerbate Inflammatory and Atherogenic Process.
Source: Public Library of Science (PLoS)
Thymoquinone, the Nigella sativa Bioactive Compound, Prevents Circulatory Oxidative Stress Caused by 1,2-Dimethylhydrazine in Erythrocyte during Colon Postinitiation Carcinogenesis
Source: Hindawi Limited
Evaluation of the effects of weak and moderate static magnetic fields on the characteristics of human low density lipoprotein in vitro.
Source: Wiley
Protection of endogenous β-carotene in LDL oxidized by oxygen free radicals in the presence of supraphysiological concentrations of melatonin
Source: Informa UK Limited
Free radicals and antioxidants: a personal view.
Source: Oxford University Press (OUP)
Dapsone protects brain microvascular integrity from high-fat diet induced LDL oxidation
Source: Springer Science and Business Media LLC
Endothelial and smooth muscle cells alter low density lipoprotein in vitro by free radical oxidation.
Source: Ovid Technologies (Wolters Kluwer Health)
Inhibition of Advanced Glycation End-Product Formation by Origanum majorana L. In Vitro and in Streptozotocin-Induced Diabetic Rats
Source: Hindawi Limited

Linking provided by 

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.