Enzymatic and Non-Enzymatic Formation of Free Radicals From Aflatoxin B₁

M. Kodama Biophysics Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo, 104; Division of Experimental Chemotherapy, Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Inohana 1-8-1, Chiba, 280, Japan

F. Inoue Biophysics Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo, 104

M. Akao Division of Experimental Chemotherapy, Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Inohana 1-8-1, Chiba, 280, Japan

Abstract

Formation of free radicals from aflatoxin B₁ was demonstrated in systems of a) NADPH/microsome, b) H₂O₂/peroxidase, c) UV irradiation, d) silver oxide oxidation and e) alkaline dimethyl sulfoxide. In a) to c), radical spots were detected in thin-layer chromatograms. Among several known metabolites of aflatoxin B₁, aflatoxin M₁ was active in generation of free radical. These results suggest a possible role of free radical metabolites in the cytotoxicity of this hepatocarcinogen.

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Enzymatic and Non-Enzymatic Formation of Free Radicals From Aflatoxin B₁

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Enzymatic and Non-Enzymatic Formation of Free Radicals From Aflatoxin B₁