Involvement of Different Transduction Pathways in NF-kB Activation by Several Inducers

Abstract

Double-stimulation was used to demonstrate that, in a T lymphocytic cell line (CEM), phorbol myristate acetate (PMA) rapidly induced NF-kB through a signaling pathway which did not involve reactive oxygen species (ROS) and was different from the activation triggered by either H₂O₂ or tumor necrosis factor-α (TNF-α). Since these latter compounds were known to activate NF-kB translocation in a redox-sensitive way, we have demonstrated that NF-kB activation by PMA was resistant to antioxidant N-acetyl-L-cysteine (NAC) and sensitive to kinase inhibitors staurosporine and H7 while activation by H₂O₂ or TNF-a were not.

Key Words:

• NF-kB
• CEM cell line
• PMA
• TNF-α
• oxidative stress