A Novel Immunoadsorbent for Rheumatoid Arthritis Therapy—Preparation and Efficacy Evaluation

Abstract

Aim To develop a novel immunoadsorbent for rheumatoid arthritis (RA) therapy. Methods: A RA immunoadsorbent was developed by binding heat-aggregated human IgG(HAHIgG) to porous agar gel beads. Its adsorption capacity for rheumatoid factors (RFs), storage stability and blood compatibility were evaluated. Results: The coupling yield of HAHIgG on the carrier was 6.0mg/g wet gel. Saturation adsorption capacity of the adsorbent for IgMRF, IgGRF and IgARF were 3400, 2240 and 2400 IU/g, respectively. The adsorbent can be stored at 4°C for three months without significant variance in its activity. Its fine permeability and hemocompatibility were demonstrated by extracorporeal hemoperfusion on rabbits. Conclusion: HAHIgG/agar gel is a safe and effective immunoadsorbent for RA therapy, its potential clinical use is promising in the future.

Key Words:

• immunoadsorbent
• heat aggregated human IgG
• rheumatoid arthritis
• rheumatoid factors