Cellular volume determination of alginate-entrapped hepatocytes by MRI diffusion measurements

G. Capuani Research labs, Sigma Tau, Pomezia, Rome, Italy

A. Miccheli Department of Chemistry, University “La Sapienza”, P.le A. Moro 5, 00185, Rome, Italy

A. Tomassini Department of Chemistry, University “La Sapienza”, P.le A. Moro 5, 00185, Rome, Italy

L. Falasca Department of Cellular and Development Biology, University “La Sapienza”, P.le A. Moro 5, 00185, Rome, Italy

T. Aureli Research labs, Sigma Tau, Pomezia, Rome, Italy

F. Conti Department of Chemistry, University “La Sapienza”, P.le A. Moro 5, 00185, Rome, Italy

Abstract

Cellular volume of hepatocytes entrapped in alginate gel beads were evaluated under in vivo conditions in samples having different cell densities by applying matemathical models to the diffusion data obtained by magnetic resonance imaging (MRI). The calculated average volume is in good agrement with the values from the literature - being closer to the data relative to living tissue than to isolated cells. The non invasive characteristics of magnetic resonance imaging make this method particularly well suited to obtain information from the intact system.

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Cellular volume determination of alginate-entrapped hepatocytes by MRI diffusion measurements

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Cellular volume determination of alginate-entrapped hepatocytes by MRI diffusion measurements

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