Inhibition of the Osteoclast Activity with the Application of Recombinant Murine RANK Protein

Abstract

Objective: To investigate the effects of recombinant Murine RANK on the osteoclast activity. Methods: Osteoclast was observed with soluble RANK. Female Wistar rats were bilaterally ovariectomized, and intra-abdominally injected with 5 mg/Kg soluble RANK. The bone metabolism, bone density, and bone histomorphology were observed. Results: Compared with the control group, the quantity of TRAP-positive osteoclasts and bone resorption pit counting in the rest groups were significantly reduced. The bone density of the dosed groups was significantly increased and TRAP-stained osteoclasts in bone tissue sections were almost inhibited. Conclusion: rh-Murine RANK was able to inhibit osteoclast differentiation and prevent ovariectomy-induced bone loss.

Keywords::

• osteoclasts
• recombinant Murine RANK protein
• ovariectomy
• osteoporosis

Declaration of Interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.