Nonthrombogenic Surface by Radiation Grafting of Heparin: Preparation, in-vitro and in-vivo Studies

Abstract

This is a study of the feasibility of radiation grafting of heparin to cellulose membranes. No significant amount of heparin could be bound to cellulose nitrate or cellulose acetate membranes by gamma radiation. Two-hour irradiated DEAECA membranes retained the highest amount of heparin; however, there was a 25% decrease in tensile strength. In-vitro studies show that blood did not clot even after 60 min on this membrane. In-vivo studies in dogs showed a significantly higher thrombo-resistance on the heparanized samples as compared to control samples.