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Abstract
Surgically removed organs were perfused with oxygenated perfluorochemical blood substitute(FDA-O2). The perfusion was carried out using resected mouse liver. ATP level of perfused liver was remaining normal until 80 min. ATP peak disappeared when perfusion was discontinued, and it reappeared on reperfusion. No significant ischemic changes were demonstrated. Ex-vivo autoradiogram successfully labelled the S phase cells in various organs and various diseases. The absorptive study is also performed. Ex-vivo organ perfusion with FDA-O2 is able to keep the resected human organs viable, and is valuable new method to analize the function and histogenesis of pathology.