6
Views
12
CrossRef citations to date
0
Altmetric
Original Article

Bilirubin Removal by the Pseudoperoxidase Activity of Free and Immobilized Hemoglobin and Hemoglobin Co-Immobilized with Glucose Oxidase

, &
Pages 553-562 | Published online: 11 Jul 2009
 

Abstract

We report a process for oxidizing bilirubin. Hemoglobin in the presence of an oxidizing agent can effectively oxidize bilirubin. Hemoglobin can be used either in free form or immobilized. Immobilisation includes microencapsulation, covalent linkage to carriers, intermolecular crosslinking into polyhemoglobin and others. The oxidizing agent can be added in the form of a peroxide. However, no external peroxide is required when hemoglobin is co-immobilized with glucose oxidase. In this case, the oxidation reaction takes place in the presence of glucose which is normally available in the blood stream and the presence of other oxidizing agents becomes unnecessary. In cases where bilirubin is conjugated to large proteins such as albumin, oxidation can be accomplished by using hemoglobin either in free form, adsorbed or bound on a suitable surface. Polyhemoglobin or cross-linked hemoglobins can also be used as oxidation catalysts in this case.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.