New NMR Probe Designs for Neonatal, Immature and Adult Heart Research with a Brief Review

Abstract

We present three new radiofrequency probes for nuclear magnetic resonance (NMR) research with perfused rabbit hearts at different maturational ages. The objective of the double-tunable, cylindrical-window probe design was to achieve a highly homogeneous magnetic field throughout the 16, 25 or 30 mm diameter usaole volume for consistency of comparison of measurements obtained from neonate, immature and adult rabbit hearts, respectively. This probe design tunes to 23-Sodium for rapid shimming and then, to 31-Phosphorus for measurements of pH and high energy phosphate metabolites. All three probes yielded excellent signal-to-noise ratios and radiofrequency operating characteristics. We introduce these new probes here in the context of a brief review of other state-of-the-art designs for in vitro and in vivo cardiovascular research.