Pretreatment Before Ischemia Induction with Polymerized Human Placenta Hemoglobin (PolyPHb) Attenuates Ischemia/Reperfusion Injury-induced Myocardial Apoptosis

Abstract

This study was designed to investigate the influence of polymerized human placenta hemoglobin (PolyPHb) pretreatment on ischemia/reperfusion (I/R) injury-induced myocardial apoptosis. Isolated Sprague-Dawley rat hearts were perfused by Langendorff model. After basal perfusion, hearts were arrested by St.Thomas’ solution (STS) with (Pre-HBOC group) or without PolyPHb (STS group), then subjected to I/R injury. Our results showed that PolyPHb pretreatment greatly reduced the TUNEL-positive myocardial cells and decreased the caspase-3 activity and cleavage, which was further confirmed by the linear regression analysis. Therefore, PolyPHb pretreatment was beneficial to attenuation of myocardial apoptosis and provided protection to the heart against I/R injury.

Keywords::

• hemoglobin-based oxygen carrier
• pretreatment
• myocardial apoptosis
• ischemia/reperfusion injury

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.