A Simple and Rapid Procedure for Purification of Haptoglobin from Human Plasma Fraction IV

Lijing Sun National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P.R. China; Graduate University of Chinese Academy of Sciences, Beijing, P.R. China

Yongdong Huang National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P.R. China

Yan Zhang National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P.R. China; Graduate University of Chinese Academy of Sciences, Beijing, P.R. China

Qingqiang Meng National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P.R. China; Graduate University of Chinese Academy of Sciences, Beijing, P.R. China

Jian Luo National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P.R. China

Bei Fan Hualan Biological Engineering Inc. Xinxiang, P.R. China

Guanghui Ma National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P.R. China

Zhiguo Su National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P.R. ChinaCorrespondence[email protected]

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Abstract

Human plasma fraction IV is an intermediate precipitate during the production of human serum albumin using cold ethanol method. Haptoglobin locates in this fraction can be purified for various applications. A new process integration of polyethylene glycol (PEG) precipitation and ion-exchange chromatography (IEC) was developed for purification of haptoglobin, which could effectively purify the haptoglobin from 16.6% to 95%. The recovery of the new process was 58.2% in comparison to 30.3% of the conventional affinity chromatography. Furthermore, 175 mg haptoglobin production in a scaled-up process showed the method to be simple, fast, and low-cost.

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Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

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A Simple and Rapid Procedure for Purification of Haptoglobin from Human Plasma Fraction IV

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A Simple and Rapid Procedure for Purification of Haptoglobin from Human Plasma Fraction IV

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