Microencapsulated Mammalian Cells for Simultaneous Production of VEGF₁₆₅b and IFNα

Abstract

Abstract: Targeted and simultaneous delivery VEGF165b and IFN alpha in anti-angiogenic and other applications could offer several advantages. For this a system was design using artificial cell alginate-poly-L-lysine- alginate (APA) microcapsules. Result confirms the ability of this system for simultaneous production of these proteins for 28-days. The IFN alpha on a 3 days period increased from 8 ± 0.36 μg/ml at day 10 to 27 ± 2.4 μg/ml at day 16 and then dropped to 6.5 ± 0.5 μg/ml. The VEGF165b on a 3 days period increased from 2.7 ± 0.7 μg/ml at day 10 to 6.9 ± 1 μg/ml at day 16.

Keywords::

• artificial cell
• microcapsule
• co-encapsulation
• angiogenesis
• IFNα
• VEGF₁₆₅b
• alginate
• poly-L-lysine
• cell therapy

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.