Polyhemoglobin-superoxide Dismutase-catalase-carbonic Anhydrase: A Novel Biotechnology-based Blood Substitute that Transports Both Oxygen and Carbon Dioxide and Also Acts as an Antioxidant

Abstract

Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase (PolyHb-SOD-CAT-CA) is a therapeutic antioxidant that also transports both oxygen and carbon dioxide. This is formed by crosslinking Hb with SOD, CAT, and CA using glutaraldehyde. Crosslinking stroma-free Hb from red blood cell (RBC) reduces CA activity to 55%. Addition of more CA resulted in a preparation with the same CA activity as RBC. PolyHb in the complex acts as a buffer to prevent large pH changes as carbon dioxide is converted to carbonic acid. We then prepare and optimize a novel PolyHb-SOD-CAT-CA, a therapeutic antioxidant that also transports both oxygen and carbon dioxide.

Keywords::

• Blood substitute
• polyhemoglobin
• carbonic anhydrase
• superoxide dismutase
• -catalase
• PolyHb-CA
• PolyHb-SOD-CAT-CA
• Carbon dioxide transport
• antioxidant
• nanobiotechnology

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

NOTICE OF CORRECTION

If you want to cite the true citation please use the following: Artificial Cells, Blood Substitutes, and Biotechnology, 39:127–136.