Immobilization of paraoxonase onto chitosan and its characterization

Abstract

Paraoxonase was covalently immobilized onto a glutaraldehyde containing amino group functionalized chitosan surface by chemical immobilization at pH 8.0. The amount of covalently bound hPON1 was found to be 32 mg/10 chitosan beads. The properties of immobilized enzyme were investigated and compared to those of free enzyme. The effects of various parameters such as pH, temperature, heat, and storage stability on immobilized enzyme were investigated. Kinetic parameters of the immobilized enzyme were also evaluated. Thermal and storage stability experiments were carried out. It was observed that the immobilized enzyme had longer storage stability and retained 50 % of its initial activity during 26 days.

Keywords::

• Chitosan
• hPON1
• purification
• covalent immobilization

Acknowledgement

The authors most gratefully acknowledge Balikesir University Research Project for the financial support of this work (Project number 2009/02).

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.