Nanomaterial-based composite biosensor for glucose detection in alcoholic beverages

Abstract

A composite electrode was prepared by modifying glassy carbon micro particles with gold nanoparticles and glucose oxidase enzyme for glucose detection. In terms of analytical characteristics, a linearity was obtained in the concentration range between 50.0 and 1000.0 µM with equation of y = 0.0015x + 0.2336 with the correlation coefficient of R² = 0.9801. R.S.D. value was calculated for 400 µM glucose (n = 6) and found to be 4.89%. LOD and LOQ values were also calculated and revealed to be 52.9 µM and 176.3 µM, respectively. The developed system was also applied for detection of glucose in four types of alcoholic beverages.

Keywords::

• Gold nanoparticles
• alcoholic beverages
• glassy carbon paste electrode
• glucose