Comparative diffusivity measurements for alginate-based atomized and inkjet-bioprinted artificial cells using fluorescence microscopy

Maryam Mobed-MiremadiDepartment of Biomedical, Chemical and Materials Engineering, Davidson College of Engineering, San Jose State University, San Jose, CA, USACorrespondence[email protected]

Behrokh AsiDepartment of Biomedical, Chemical and Materials Engineering, Davidson College of Engineering, San Jose State University, San Jose, CA, USA

Jaino ParasserilDepartment of Biomedical, Chemical and Materials Engineering, Davidson College of Engineering, San Jose State University, San Jose, CA, USA

Emily WongDepartment of Biomedical, Chemical and Materials Engineering, Davidson College of Engineering, San Jose State University, San Jose, CA, USA

Man TatDepartment of Biomedical, Chemical and Materials Engineering, Davidson College of Engineering, San Jose State University, San Jose, CA, USA

Yiming ShanDepartment of Biomedical, Chemical and Materials Engineering, Davidson College of Engineering, San Jose State University, San Jose, CA, USA

Abstract

Radial diffusivity profiles of atomized (MC, d = 1800 ± 200 µm) and inkjet-printed (MI, d = 40 ± 5 µm) alginate-based artificial cells have been generated using 2D Fluorescence Microscopy. The passive outward diffusion of FITC-Dextrans from MIs (0.5% LV alginate/15% CaCl₂ coated with 0.5% Chitosan) and MCs (1.5% MV alginate/1.5% CaCl₂) was measured and quantified using a Fickian model. As an expected outcome of miniaturization, the ratios of the outer layer diffusivities defined as D_MIout/D_MCout were 4.25 and 5.07 respectively for the 4 and 70 kDa markers, indicative of the enhanced diffusive potential of the miniaturized capsules.

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Comparative diffusivity measurements for alginate-based atomized and inkjet-bioprinted artificial cells using fluorescence microscopy

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Comparative diffusivity measurements for alginate-based atomized and inkjet-bioprinted artificial cells using fluorescence microscopy

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