Properties of immobilized glucose oxidase and enhancement of enzyme activity

Abstract

In this study; glucose oxidase (GOD) and MnO₂ were immobilized in alginate gel beads using the entrapment method and effects of oxygen vectors on immobilized GOD activity were investigated. The specific activity of free enzyme was 1.3 units/mg protein whereas that of immobilized enzyme was 8.4 × 10⁻¹ units/mg protein. The Km values for free and immobilized enzyme were found to be 2.7 mM, 2.9 mM; Vmax values 3.64 × 10⁻¹ μmol/min.mL, 2.61 × 10⁻¹ μmol/min.mL, respectively. When 4% (v/v) silicone oil or soybean oil was used, the activity of immobilized GOD increased 1.7 times and 1.3 times, respectively, compared to media without these oils.

Keywords::

• calcium
• alginate gel
• gluconic acid
• immobilized glucose oxidase
• silicone oil
• soybean oil

Declaration of interest The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.