Biospecific Adsorbents on the Basis of Chloroformate-Activated Bead Cellulose

C. Rupprich Academy of Sciences of the GDR, Central Institute of Molecular Biology, Department of Chemistry, Berlin, GDR

M. Becker Academy of Sciences of the GDR, Central Institute of Molecular Biology, Department of Chemistry, Berlin, GDR

W. Büttner Academy of Sciences of the GDR, Central Institute of Molecular Biology, Department of Chemistry, Berlin, GDR

H.-F. Boeden Academy of Sciences of the GDR, Central Institute of Molecular Biology, Department of Chemistry, Berlin, GDR

K.-L. Schröder Academy of Sciences of the GDR, Central Institute of Molecular Biology, Department of Chemistry, Berlin, GDR

A. Hänsicke Karl Marx University, Department of Biochemistry, Leipzig, GDR

A. Könnecke Karl Marx University, Department of Biochemistry, Leipzig, GDR

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Abstract

Carbohydrate-derived polymers are activated by the chloroformate N-chlorocarbonyloxy-5-norbornene-2.3-dicarboxlmide (CICOONB). The advantages of this activation method are presented. The application of bead cellulose as adsorbent for biomedical and biotechnological purposes is demonstrated. Examples for immunoglobulin purification, streptavidin isolation, and biotransformation of porcine insulin are given.

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Biospecific Adsorbents on the Basis of Chloroformate-Activated Bead Cellulose

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Biospecific Adsorbents on the Basis of Chloroformate-Activated Bead Cellulose

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