Hepatic Reticuloendothelial Function Following Resuscitation with Hemoglobin Solutions

Abstract

Red cell substitutes could lead to depressed reticulo-endothelial (RE) particulate clearance function. This hypothesis was tested using an animal model of hypovolemia-resuscitation. Anesthetized male Sprague-Dawley rats were subjected to 50% blood volume hemorrhage followed by isovolumic replacement with stroma-free hemoglobin (SFH, 7gHb/dl), polyhemoglobin (PHS, 14gHb/d1), or shed blood (SB). At 30min post-transfusion, the liver was isolated and perfused with colloidal carbon. Hepatic RE function was assessed from the carbon clearance kinetics. In separate experiments, the hepatic Kupffer cells were isolated and cultured from rats that were previously hemorrhaged and transfused with normal saline solution. The cultured Kupffer cells were incubated with SFH or bovine albumin (ALB) and their phagocytic function assessed in-vitro. The hepatic carbon clearance following exchange transfusion with hemoglobin solutions was not significantly altered as compared to shed blood controls (P>0.05). Similarly, phagocytic function of hemoglobin treated Kupffer cells was not significantly different (P>0.05) from that of ALB treated cells.