Controlled Release of Proteins from 2-Hydroxyethyl Methacrylate Copolymer Gels

Abstract

A series of hydrogels with large pores was synthesized by the precipitation polymerization of 2-hydroxyethyl methacrylate (HEMA) with crosslinking agent in aqueous solution. Such gels are potentially useful for the controlled release of large-molecular-weight species such as proteins. In this study, the release behavior of lysozyme and α-amylase from hydrogels formed from HEMA or HEMA with a comonomer was studied. It was found that the polymer composition affected the total amount of lysozyme released and its activity. Effects were smaller with α-amylase. Charged gels, containing a phosphate moiety, released larger amounts of lysozyme at a reduced rate as a result of charge-charge interactions.