Prolongation of the Intravascular Retention of Hemoglobin Modified with a Long-Chain Fatty Acid Derivative

Scott A. Fowler Department of Biochemistry, College of Medicine, Iowa City, Iowa, 52242

Mark Andracki Department of Biochemistry, College of Medicine, Iowa City, Iowa, 52242

Gerald Hurst Department of Biochemistry, College of Medicine, Iowa City, Iowa, 52242

Vidya A. Honkan Department of Biochemistry, College of Medicine, Iowa City, Iowa, 52242; Division of Medicinal and Natural Products Chemistry, College of Phannacy The University of Iowa, Iowa City, Iowa, 52242

Joseph Waldorf Department of Biochemistry, College of Medicine, Iowa City, Iowa, 52242

Dee Ann Casteel Division of Medicinal and Natural Products Chemistry, College of Phannacy The University of Iowa, Iowa City, Iowa, 52242

Abstract

To develop hemoglobin (Hb) derivatives with an increased circulatory half-life, Hb was chemically modified with long chain fatty acid analogs. One compound, sodium 1-hexadecyl 6-(2-iodoacetamido)hexyl phosphate, specifically modified the Cys-93β residues of human hemoglobin (HbA) as determined by sulfhydryl titration analysis. The resulting modified Hb derivative, FAHbA, was isolated and was shown to have a two-fold longer circulatory half-life than native HbA in a rat low-dose acute transfusion model.

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Prolongation of the Intravascular Retention of Hemoglobin Modified with a Long-Chain Fatty Acid Derivative

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Prolongation of the Intravascular Retention of Hemoglobin Modified with a Long-Chain Fatty Acid Derivative

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