Normothermic Preservation of “Multiple Organ Blocks” with a New Perfluorooctyl Bromide Emulsion

Abstract

To evaluate the efficiency of fluorocarbon emulsions as oxygenating media for the normothermic preservation of organs (multiple organ blocks, MOB), a new perfluorooctyl bromide (perflubron) emulsion was compared with a mixture of modified Krebs solution and blood. The fluorocarbon emulsion used (90% w/v of fluorocarbon) contained a low amount of egg yolk phospholipid (EYP, 2% w/v) and was stabilized by a mixed fluorocarbon-hydrocarbon amphiphile C₆F₁₃C₁₀H₂₁ (F6H10). Blood of 4 rat MOBs was replaced with a 36% w/v fluorocarbon emulsion which had been complemented with albumin and electrolytes (EMOBs). 5 MOBs were perfused with a mixture of blood and albumin-containing Krebs solution (KBMOBs). Lactate, amylase and creatine kinase were lower (p < 0.05) at 60 and 120 min in EMOBs than in KBMOBs, showing lesser suffering of the organs. Diuresis was also higher (p < 0.05) in EMOBs (5.65±1.76 vs 1.21±0.28 mg/min). There was no difference in bile production, AST, ALT levels. Electrolytes and acid-base balance were preserved in all cases; PaO₂ was significantly higher (p < 0.05) for the EMOBs than for the KBMOBs. It is concluded that fluorocarbon emulsions provide significant improvement in the normothermic preservation of organs.