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Abstract
Protoplasts from cell suspensions of albino Petunia hybrida cv. Comanche were cultured for 9 days in nutrient medium containing Erythrogen™, a purified bovine haemoglobin solution (supplied at 10% w/v) at 1:50 - 1:500 (v/v). In some assessments, the non-ionic surfactant Pturonic® F-68 (Poloxamcr 188), was also added to the culture medium at 0.01-1.0% (w/v). Erythrogen™ at 1:50 (v/v) increased the mean initial protoplast plating efficiency (IPE; 18.5 + 0.8%, n = 5 throughout) by 64% (P < 0.001) above that of controls (11.3 ± 0.4%). Supplementation of medium with 1:50 (v:v) Erythrogen™ and 0.01% (w/v) Pluronic® F-68, increased the mean IPE (24.4 ± 1.4%) by 92% (P < 0.001) over control (12.7 ± 1.1%). Similar results were obtained for mesophyll protoplasts of Passiflora suberosa, with 1:50 and 1:100 (v/v) Erythrogen™ increasing the mean IPEs to 87% and 93% respectively, over controls. This beneficial and synergistic effect of Erythrogen™ with Pluronic® F-68, on mitotic division of cultured Petunia and Passiflora protoplasts, should also facilitate the culture of isolated protoplasts and cells of other, agronomically-important, species.
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P. Anthony
Joyce Laing works in the Department of Child and Family Psychiatry, Playfield House, Cupar, Fife, and is a Consultant Art Therapist to Psychiatric Hospitals and Prisons and Chairwoman of the Scottish Society of Art and Psychology.