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Abstract
Superoxide dismutase (superoxide oxidoreductase; EC 1.15.1.1; SOD) was measured in cnzymatically isolated protoplasts of Salpiglossis sinuata following culture in aqueous nutrient medium overlaying oxygen-gassed perfluorodecalin (Flutec R PP6; BNFL Fluorochemicals, UK). SOD was extracted from harvested, lysed protoplast-derived cells after 1,3, 7 and 14 days of culture and assayed speetrophotometrically. Protoplasts cultured with oxygenated PFC (± s.e.m, n = 5) showed significant increases in mean SOD activity to 4.2 ± 0.1 U after 1 day (P < 0.05) and 9.3 ± 0.7 U after 3 days (P < 0.01), with a fall in mean SOD after 7 days (5.1 ± 0.9 U), similar to control. The decrease in SOD after 7 days correlated closely with a progressive fall in pO2 in the PFC phase over the same period. In contrast, control protoplasts (medium alone) or protoplasts cultured in medium overlaying non-oxygenated PFC showed no significant changes in mean SOD activity over the 14-day culture assessment period.
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Notes on contributors
J. Wardrop
Joyce Laing works in the Department of Child and Family Psychiatry, Playfield House, Cupar, Fife, and is a Consultant Art Therapist to Psychiatric Hospitals and Prisons and Chairwoman of the Scottish Society of Art and Psychology.